DNA Reverse Complement Calculator
Generate reverse complement sequences for PCR primers, cloning, and molecular biology experiments.
✓ Instant calculation✓ Supports IUPAC codes✓ Multiple sequences
DNA Sequence Input
0 bases
Reverse Complement
Understanding DNA Reverse Complement
What is Reverse Complement?
The reverse complement is the sequence that would pair with your input sequence in double-stranded DNA, read in the opposite direction.
5'-ATCGATCG-3' (original)
3'-TAGCTAGC-5' (complement)
5'-CGATCGAT-3' (reverse complement)
Base Pairing Rules
Standard Bases:
A ↔ T
T ↔ A
G ↔ C
C ↔ G
IUPAC Codes:
R ↔ Y
S ↔ S
W ↔ W
K ↔ M
Common Applications
PCR Primer Design
Design reverse primers that bind to the complement strand for PCR amplification.
Cloning & Subcloning
Determine sequences for both strands when designing cloning strategies.
Sequence Analysis
Analyze both strands for features like ORFs, restriction sites, or motifs.