GC Content Calculator
Analyze sequence composition and calculate GC content percentage for DNA and RNA sequences.
✓ Multiple sequences✓ Detailed composition✓ Quality indicators
DNA/RNA Sequence Input
0 total bases
Analysis Results
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Understanding GC Content
What is GC Content?
GC content is the percentage of bases in a DNA or RNA sequence that are either guanine (G) or cytosine (C).
- • Higher GC content = more stable DNA
- • G-C pairs have 3 hydrogen bonds
- • A-T pairs have only 2 hydrogen bonds
- • Affects melting temperature and PCR conditions
GC Content Ranges
Low (<40%)
Less stable, lower Tm
Optimal (40-60%)
Balanced stability
High (>60%)
Very stable, higher Tm
Applications of GC Content Analysis
PCR Design
Primers with similar GC content ensure even amplification and optimal annealing temperatures.
Genome Analysis
Different organisms have characteristic GC content patterns that aid in classification.
Cloning Strategy
High GC sequences may require special vectors or expression conditions.